Different patterns and functional importance of correlation between adjacent alternative exons in human and mouse
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چکیده
We re-sampled and permutated the EST evidence to make sure of two things: 1) the bias toward positive values in the distribution of correlation coefficient is not an artifact of small, discrete value of EST numbers; 2) the peaks on-1 and +1 in the distribution are not artifacts of biased distribution of correlation coefficients. These procedures were applied to human. To address the first question, a re-sampling procedure was employed. First we constructed 3003 hypothetical pairs (the final number of human adjacent alternative pairs in our analysis) and the number of ESTs covering each pair was re-sampled from the true distribution of EST number (Figure S1). Then, we re-sampled the exon inclusion levels for upstream and downstream exons in each pair (Figure S4). By assuming that two exons in a pair are independent, the probability of the four types of ESTs (Figure 1) can be determined by taking the product of the marginal distributions. We sampled the ESTs from this joint distribution. Thus, for each hypothetical pair, a correlation coefficient can be obtained and the 3003 new correlation coefficients result in a re-sampled distribution. Figure S2A is an example of such a distribution. It is zero-centered, so the bias towards positive values is an intrinsic nature of the observed distribution of correlation
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تاریخ انتشار 2008